Antibody Panels and Kits

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MilliporeSigma™ RIPAb+™ hnRNP U RIP Validated Antibody and Primer Set

This RIPAb+ hnRNP U -RIP Validated Antibody and Primer Set conveniently includes the hnRNP U antibody and the specific control PCR primers.

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BD Mouse Naive/Memory T Cell Panel

Contains fluorescent antibodies specific for CD44, CD62L, CD4 and CD3 cell surface antigens

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BD Biosciences IMag Lymphocyte Enrichment Set - DM , Clone: M1/70, RA3-6B2, HMα2, TER-119, BD

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MilliporeSigma™ Aquaporin 2 (254-271), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Rabbit Polyclonal Antibody

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Specifications

Includes	50μL antibody lyophilized from PBS, 5% sucrose, pH 7.4 and 40μg control peptide lyophilized from PBS.
Antigen	Aquaporin 2 (254-271)
Regulatory Status	RUO
Content And Storage	−20°C
Host Species	Rabbit
Applications	Immunohistochemistry,Immunoprecipitation
Form	Purified
Formulation	50μl antibody lyophilized from PBS, 5% sucrose, pH 7.4 and 40μg control peptide lyophilized from PBS.
Immunogen	a synthetic peptide [(KY)RQSVELHSPQSLPRGSKA] corresponding to amino acids 254-271 of rat and mouse AQP2,conjugated to KLH
Classification	Polyclonal
Isotype	IgG
Primary or Secondary	Primary
Test Specificity	Recognizes the ∽40kDa and ∽29kDa forms of aquaporin-2 in rat kidney membrane. Supplied with a control peptide (AQP2_254-271 immunogen peptide).

BD Biosciences Dog Compensation Set, Clone: LSM 1.140, LSM 12.125, LSM 8.358, CTL 2.58 , BD

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BD B Lymphocyte Subset Antibody Cocktail

Three-color reagent designed to identify major subsets of B lymphocytes

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MilliporeSigma™ Upstate™ Estrogen Receptor αα, Mouse monoclonal, ChIP Validated Antibody and Primer Set

Mouse Monoclonal Antibody

Pricing & Availability
Specifications

Content And Storage	−20°C for one year from date of shipment
Host Species	Mouse
Applications	ChIP Assay,Western Blot
Form	Ascites
Gene Accession No.	P03372
Includes	This ChIPAb+ Estrogen Receptor α -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Antigen	Estrogen Receptor αα
Regulatory Status	RUO
Gene Symbols	ESR1, Era, ER-alpha, ESR, ER, NR3A1, ESRA, DKFZp686N23123
Gene ID (Entrez)	NM_000125.2
Formulation	Anti-ERα (mouse ascites). 1 vial containing 100mL ascites. The ERα antibody is made against aa120-170 of bovine estrogen a. It can recognize human and mouse ERα. ChIP primers TFF1 (pS2). 1 vial containing 75mL of 5μM of each control primer specific for human TFF1 (pS2) promoter. FOR: CCG GCC ATC TCT CAC TAT GAA; REV: CCT TCC CGC CAG GGT AAA TAC
Immunogen	The ERα antibody is made against aa 120-170 of bovine estrogen α.
Classification	Monoclonal
Primary or Secondary	Primary
Test Specificity	Estrogen Receptor α

MilliporeSigma™ Upstate™ Monomethyl-Histone H3 (Lys27), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Rabbit Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	−20°C from date of receipt
Host Species	Rabbit
Applications	ChIP Assay,Electromobility Shift Assay,Flow Cytometry,Immunofluorescence,Immunohistochemistry,Western Blot
Form	Serum
Gene Accession No.	Q66I33
Includes	This ChIPAb+ Monomethyl-Histone H3 (Lys27) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Antigen	Monomethyl-Histone H3 (Lys27)
Regulatory Status	RUO
Gene Symbols	H3F3A, H3F3B, H3F3
Gene ID (Entrez)	NM_003493
Formulation	Anti-monomethyl-Histone H3 (Lys27) (rabbit polyclonal IgG). One vial containing 100μL of antiserum containing 0.05% sodium azide. Normal Rabbit Serum. One vial containing 100μL antiserum containing 0.05% sodium azide. ChIP Primers, β-Globin. One vial containing 75μL of 5μM of each primer specific for for human β-globin promoter. FOR: AGG ACA GGT ACG GCT GTC ATC REV: TTT ATG CCC AGC CCT GGC TC
Immunogen	The monomethyl-histone H3 (Lys27) antiserum is made against a synthetic 2X-branched peptide containing the sequence…ARmeKSA…in which meK corresponds to monomethyl-lysine 27 of human histone H3.
Classification	Polyclonal
Primary or Secondary	Primary
Test Specificity	Monomethyl-Histone H3 (Lys27)

BD Biosciences Mouse IL-10 ELISPOT Pair, BD

Convenient set of unlabelled anti-IL-10 capture antibody and a biotinylated anti-IL-10 detection antibody

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MilliporeSigma™ Upstate™ CTCFα, Mouse monoclonal, ChIP Validated Antibody and Primer Set

Mouse Monoclonal Antibody

Pricing & Availability
Specifications

Content And Storage	-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Host Species	Mouse
Applications	Multiplex,Dot Blot,ELISA,Immunohistochemistry,Immunoprecipitation,Immunoassay,Western Blot
Form	Purified
Gene Accession No.	P49711
Isotype	IgG1 κ
Includes	The ChIPAb+ CTCF set includes the CTCF antibody, a negative control antibody (normal mouse IgG) and qPCR primers which amplify a 165bp region of human H19 internal control region.
Antigen	CTCFα
Regulatory Status	RUO
Gene Symbols	CTCF
Purification Method	Protein G purified
Gene ID (Entrez)	NP_006556.1
Formulation	Anti-CTCF, (mouse monoclonal IgG1κ. One vial containing 50μg of protein G purified mouse monoclonal IgG1κ in buffer containing 0.1M Tris-Glycine (pH 7.4, 150mM NaCl) with 0.05% sodium azide, before the addition of glycerol to 30%. Normal Mouse IgG. Two vials containing 25μg purified IgG in 25μL storage buffer containing 0.1% sodium azide. ChIP Primers, Human H19 ICR. One vial containing 75μL of 5μM of each primer specific for human H19 internal control region. FOR: CCC ATC TTG CT GACC TCAC; REV: AGA CCT GGG ACG TTT CTG TG
Immunogen	His-tagged recombinant protein corresponding to human CTCF.
Classification	Monoclonal
Primary or Secondary	Primary
Test Specificity	Recognizes CTCF, Mr ∽130kDa.

MilliporeSigma™ Upstate™ Dimethyl-Histone H3 (Lys36), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Rabbit Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Host Species	Rabbit
Applications	ChIP Assay,Electromobility Shift Assay,Immunoprecipitation,Western Blot
Form	Serum
Gene Accession No.	Q16695
Includes	This ChIPAb+ Validated Antibody and Primer Set conveniently includes the antibody, matched IgG negative control antibody and set control PCR primers that detect a known positive locus.
Antigen	Dimethyl-Histone H3 (Lys36)
Regulatory Status	RUO
Gene Symbols	H3.4, H3/g , H3/t, H3FT, H3T, H3t, MGC126886, MGC126888, OTTHUMP00000037945
Gene ID (Entrez)	NP_003484
Formulation	Anti-Dimethyl Histone H3 (Lys36) (Rabbit Polyclonal). One vial containing 25μL rabbit polyclonal serum containing 0.05% sodium azide, before the addition of 30% glycerol. Normal Rabbit Serum. One vial containing 25μL antiserum containing 0.05% sodium azide. ChIP Primers, GAPDH coding D2. One vial containing 75μL of 5μM of each primer specific for human GAPDH coding region. (chr12:6647453+6647539, hg 19 build). FOR: 5’ GCC ATG TAG ACC CCT TGA AGA GC 3’; REV: 5’ ACT GGT TGA GCA CAG GGT ACT TTA T 3’
Immunogen	KLH-conjugated linear peptide corresponding to a region near the N-terminus of human Histone H3 dimethylated at Lys36.
Classification	Polyclonal
Primary or Secondary	Primary
Test Specificity	Recognizes Dimethyl Histone H3 (Lys36). No detectable cross-reactivity unmethylated, mono, or dimethylated Lys36. No detectable cross reactivity to other histone modifications as measured by AbSurance Histone Antibody Specificity Arrays.

MilliporeSigma™ Upstate™ Acetyl-Histone H4 (Lys5)α, Rabbit Monoclonal, ChIP Validated Antibody and Primer Set

Rabbit Monoclonal Antibody

Pricing & Availability
Specifications

Content And Storage	-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Host Species	Rabbit
Applications	ChIP Assay,Immunoprecipitation,Western Blot
Form	Culture Supernatant
Gene Accession No.	P62805
Isotype	IgG
Includes	This ChIPAb+ Acetyl-Histone H4 (Lys5) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Antigen	Acetyl-Histone H4 (Lys5)α
Regulatory Status	RUO
Gene Symbols	HIST1H4A, H4/J, HIST1H4F, HIST1H4L, H4FN, H4FA, H4FH, HIST1H4H, H4F2, H4FM, H4/A, H4FK, H4FG, HIST2H4, H4FB, H4/K, HIST1H4I, H4/N, HIST1H4B, H4FE, H4/M, H4/a, HIST1H4E, HIST4H4, HIST2H4A, H4FC, H4FI, H4/H, HIST1H4C, H4/G, HIST1H4K, H4FJ, H4FD, H4/I, H4/B, H4/D, H4/C, HIST1H4J, HIST1H4D, H4/E
Gene ID (Entrez)	NP_001029249
Formulation	Anti-Acetyl-Histone H4 (Lys5) (rabbit monoclonal IgG). One vial containing 50μL of rabbit monoclonal IgG cell culture supernatant in 0.1% sodium azide. Negative Control Supernatant (rabbit). One vial containing 50μL of cultured supernatant in 0.05% sodium azide. Control Primers. One vial containing 75μL of 5μM each primer specific for the human GAPDH promoter. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA
Immunogen	Peptide corresponding to Histone H4 containing the sequence [GRG-AcK-GGK] on which Lys5 is acetylated.
Classification	Monoclonal
Primary or Secondary	Primary
Test Specificity	Recognizes Histone H4 acetylated on Lys5.

MilliporeSigma™ Upstate ChIPAb+ Dimethyl-Histone H3 (Lys9) - ChIP Validated Antibody and Primer Set

Dimethyl-histone H3 (Lys9) purified antibody is made against synthetic peptide (dimethylated at Lys9) corresponding to amino acids 1-18 of histone H3

Pricing & Availability

MilliporeSigma™ Upstate™ Acetyl-Histone H3 (Lys27)α, Mouse monoclonal, ChIP Validated Antibody and Primer Set

Mouse Monoclonal Antibody

Pricing & Availability
Specifications

Content And Storage	−20°C for up to 12 months; Avoid repeated freeze/thaw cycles
Host Species	Mouse
Applications	ChIP Assay,Western Blot
Form	Purified
Gene Accession No.	Q66I33
Isotype	IgG
Includes	Acetyl-Histone H3 (Lys27) ChIP validated Antibody and Primer set including the ChIP-grade antibody and the specific control PCR primers used for chromatin immunoprecipitation of H3K27Ac.
Antigen	Acetyl-Histone H3 (Lys27)α
Regulatory Status	RUO
Gene ID (Entrez)	NM_003493
Formulation	Anti-acetyl-Histone H3 (Lys27) (mouse monoclonal IgG1, Clone CMA309). One vial containing 50μg of protein G purified antibody in 50μL PBS containing 0.05% sodium. Normal Mouse IgG. Two vials containing 25μg purified Mouse IgG in 25μL storage buffer containing 0.1% sodium azide. ChIP Primers, RPL10 Promoter. One vial containing 75μL of 5μM of each primer specific for the promoter region of human RPL10. FOR: ACC CGT CTT CGA CAG GAC T; REV: GGA ACG GAA GAC GAG AAC AG
Immunogen	The acetyl-histone H3 (Lys27) purified antibody is made against a synthetic peptide (acetylated at Lys27) corresponding to amino acids 19-37 of histone H3.
Classification	Monoclonal
Primary or Secondary	Primary
Test Specificity	Recognizes histone H3, Mr 17kDa, acetylated at Lys27.

BD Biosciences Human IFN-γ ELISPOT Pair, BD

Convenient set of unlabelled anti-IFN-γ capture antibody and a biotinylated anti-IFN-γ detection antibody

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